Journal: Oncology Reports

Article Title: microRNA-1301-mediated inhibition of tumorigenesis

doi: 10.3892/or.2011.1589

Figure Lengend Snippet: miR-1301 inhibits the migration of HepG2 cells in a scratch test. The scratch test was used to investigate the wound healing ability at 0, 12, 24 and 48 h. We observed that the proliferation and migration ability of HepG2 cells in the wounded area was reduced in the miR-1301 group after a 24 and 48 h repair period when compared with the control group. There was no difference between the miR-1301 transfected and the control group at 12 h.

Article Snippet: Total RNA was extracted from human HepG2 cell lines using TRIzol reagent (Takara Biotechnology Co., Ltd.).

Techniques: Migration, Transfection

Journal: Oncology Reports

Article Title: microRNA-1301-mediated inhibition of tumorigenesis

doi: 10.3892/or.2011.1589

Figure Lengend Snippet: miR-1301 inhibits the invasion of HepG2 cells in the Transwell chamber assay. Cell invasion ability was analyzed by the Transwell chamber assay 48 h after miR-1301 transfection. The results showed that the number of migration cells in the miR-1301 group was less than that in control group. This indicated that the invasion ability of HepG2 cells might be inhibited by miR-1301.

Article Snippet: Total RNA was extracted from human HepG2 cell lines using TRIzol reagent (Takara Biotechnology Co., Ltd.).

Techniques: Transwell Chamber Assay, Transfection, Migration

Journal: Oncology Reports

Article Title: microRNA-1301-mediated inhibition of tumorigenesis

doi: 10.3892/or.2011.1589

Figure Lengend Snippet: miR-1301 inhibits cell proliferation. The MTT assay was performed to monitor the proliferation rate of HepG2 cells after miR-1301 transfection. The optical density of each well was measured with a microplate spectrophotometer at 490 nm. The optical density of the miR-1301 group decreased at 24 h (A), 48 h (B), and 72 h (C) after transfection. The proliferation rate of cells decreased with increasing concentrations of inhibitor and increasing transfection time (D).

Article Snippet: Total RNA was extracted from human HepG2 cell lines using TRIzol reagent (Takara Biotechnology Co., Ltd.).

Techniques: MTT Assay, Transfection, Spectrophotometry

Journal: Oncology Reports

Article Title: microRNA-1301-mediated inhibition of tumorigenesis

doi: 10.3892/or.2011.1589

Figure Lengend Snippet: miR-1301 promotes cell apoptosis. Apoptosis of HepG2 cells was observed using fluorescence microscopy through a dual pass filter allowing to visualize the Annexin-V-FITC positive and the propidium iodide positive cells in the same field. The results showed that apoptosis of HepG2 cells increased 48 h after transfection of miR-1301 mimics in the miR-1301 group (Aa) when compared with the control group (Ba). (Aa) Apoptosis cells of the Annexin-V-FITC positive cells in the miR-1301 group; (Ab) cells excited by normal light in the miR-1301 group; (Ba) apoptosis cells of the Annexin-V-FITC positive cells in the control group; (Bb) cells excited by normal light in the control group.

Article Snippet: Total RNA was extracted from human HepG2 cell lines using TRIzol reagent (Takara Biotechnology Co., Ltd.).

Techniques: Fluorescence, Microscopy, Transfection

Journal:

Article Title: Functional analysis of peroxisome-proliferator-responsive element motifs in genes of fatty acid-binding proteins

doi: 10.1042/BJ20031340

Figure Lengend Snippet: HepG2 cells were transiently co-transfected with plasmids pSG5-PPARα, -PPARβ, -PPARγ1, pcDNA3-PPARγ2 and pSG5-RXR subtypes respectively together with the pCAT3 promoter plasmid containing a PPRE and pSV-β-Gal. After 42 h, cells were harvested. β-Gal and CAT concentrations were determined by ELISAs; experiments with empty pCAT3 plasmid served as control and were set to unity (neg.). Results are means±S.D. for two independent experiments, each performed in triplicate (n=6).

Article Snippet: Reporter gene assay in HepG2 cells For experiments shown in Figure , HepG2 cells (A.T.C.C., HB-8065 or Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH, Braunschweig, Germany, ACC 180) were grown to 60–70% confluency in 6-well dishes (Nunc, Wiesbaden, Germany) in Dulbecco's modified Eagle's medium supplemented with 10% basal-medium-supplement artificial medium (both from Biochrom, Berlin, Germany).

Techniques: Transfection, Plasmid Preparation, Control

Journal:

Article Title: Functional analysis of peroxisome-proliferator-responsive element motifs in genes of fatty acid-binding proteins

doi: 10.1042/BJ20031340

Figure Lengend Snippet: (A) HepG2 cells were transiently co-transfected with pCAT3 promoter plasmid containing the PPRE, β-Gal, without and with plasmids for PPARγ1 and RXRγ. After 42 h, cells were harvested, and β-Gal and CAT concentrations were determined by ELISAs. For each PPRE, experiments were normalized to those without ectopic PPARγ1/RXRγ, set to unity. Results are means±S.D. for two independent experiments, each performed in triplicate (n=6). (B) HepG2 cells were transiently co-transfected with pCAT3 promoter plasmid containing the PPRE and plasmids for β-Gal, PPARγ1 and RXRγ. DMSO and DMSO plus bezafibrate respectively were added to the medium 4 h after transfection (final concentrations 1% DMSO and 100 μM bezafibrate) and cells were incubated for a further 38 h. After harvest, β-Gal and CAT concentrations were determined by ELISAs. For each PPRE, experiments with ligand were normalized to those with DMSO alone, set to unity. Results are means±S.D. for two independent experiments, each performed in triplicate (n=6).

Article Snippet: Reporter gene assay in HepG2 cells For experiments shown in Figure , HepG2 cells (A.T.C.C., HB-8065 or Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH, Braunschweig, Germany, ACC 180) were grown to 60–70% confluency in 6-well dishes (Nunc, Wiesbaden, Germany) in Dulbecco's modified Eagle's medium supplemented with 10% basal-medium-supplement artificial medium (both from Biochrom, Berlin, Germany).

Techniques: Transfection, Plasmid Preparation, Incubation

Journal: Virology Journal

Article Title: The enhanced expression of death receptor 5 (DR5) mediated by HBV X protein through NF-kappaB pathway is associated with cell apoptosis induced by (TNF-α related apoptosis inducing ligand) TRAIL in hepatoma cells

doi: 10.1186/s12985-015-0416-z

Figure Lengend Snippet: The apoptosis of Huh-7-HBX cells induced by TRAIL. a , b The expression of HBX in HepG2 cells was determined by RT-PCR and western blotting analysis. c After the cells treated with 100 ng/mL TRAIL for 24 h, the detection of apoptosis of Huh-7-HBX cells and control cells by flow cytometry

Article Snippet: In addition, increased expression of DR4 was observed in hepatoma cell line HepG2 transfected with HBV by Janssen HL et al [ ], and the observation enhance our hypothesis that HBX could increase the sensitivity of cell apoptosis induced by TRAIL receptors.

Techniques: Expressing, Reverse Transcription Polymerase Chain Reaction, Western Blot, Control, Flow Cytometry